FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128957696
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1347190.10446759222497275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG325550.036.347463
CAATCGC483150.034.2118071
AGGGCGA1005200.030.3210414
GGGCGAT331250.030.2344281
GGCGATC334850.029.8740962
TGCGACA585350.027.8427526
GCGACAT603350.027.0845577
TACCGTA217950.026.1871199
CGGAGGG1224850.025.3258671
CGGTGGC2017300.025.1056041
AATCGCC696300.023.8722972
TGGCGCG1426550.023.812214
GCGAGGC1423900.020.9051347
GCTGCGA793850.020.687364
GATCAAT599500.020.285671
CGACATC823250.020.093568
GGGCGAG1532500.020.0675135
GTGGCGC1769300.020.0566653
ATCAATC638150.019.5797392
CTACCGT302400.019.3286138
Produced by FastQC (version 0.11.5)