FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90275293
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1416380.15689564142428206No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1335610.1479485643984562No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1134280.12564678134027213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT232150.053.702166
TCGACCG516750.051.764214145
CCGCGTT261200.047.8518037
AGACCGC265500.046.581184
CTCGCTA405500.044.7795751
CAGACCG305100.041.3775563
TCGCTAT461150.039.763392
CGCTATG500300.036.6109663
GCGTTCT394150.031.5154559
CCGGTAA126450.029.2373121
CGATCCT933450.027.40947145
CGCGTTC470450.026.3085488
ACCGCTA136250.025.2932342
CTTCGAC716950.024.798183145
GACCGCG533650.023.4699955
CCGCTAA161850.023.4198343
CGGTAAT144950.022.9323432
CGTATCG141750.022.0271193
GTGCTCT768000.021.820974145
CGCTAGA206400.020.670251
Produced by FastQC (version 0.11.5)