FastQCFastQC Report
Fri 22 Apr 2022
EGAF00005788135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90275293
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA288350.026.3377346
TACCGTA162300.025.7424769
GCGACAT303450.025.3247747
AGGGCGA543200.024.4818214
CGGTGGC1148950.024.0599961
TCAATCG131300.023.266993
TGGCGCG775200.022.8954984
CTACCGT187500.022.2144228
CAATCGC185200.022.020271
CGGAGGG648200.021.40931
GGCGATC206900.020.3236682
GGGCGAT219350.019.0694791
CGACATC417150.018.7004178
ACTACCG230000.018.503057
CGGCCGC1931050.017.985285145
GCTGCGA440450.017.743364
GTGGCGC1076750.017.497073
GGGGACC2634700.017.117151145
GGCGCGT970750.017.0835725
CGACCGT73400.016.9446721
Produced by FastQC (version 0.11.5)