FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115851932
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1944400.1678349222523108No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1860040.16055321373492504No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1717500.14824957774549674No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGA1457060.12576915851519851No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1269550.10958384362549949No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1215420.1049115003105861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG749800.056.413464145
ACCGCGT343200.056.1524246
CCGCGTT368250.052.349697
AGACCGC402550.047.4692574
CAGACCG432500.044.6742973
CTCGCTA491250.042.146541
GCGTTCT508800.037.839489
TCGCTAT547200.037.5993962
CGCGTTC525100.036.497958
GTGCTCT1079350.036.466927145
CGCTATG604450.033.9440773
GACCGCG596900.032.2545285
CGATCCT1135850.027.118206145
CTTCGAC980250.025.588875145
ACCGCTA202750.024.55082
CCGCTAA241100.021.1645153
CGCTAAG248500.020.0555444
GCTATGT1075900.019.5356184
GAGTCTT1425650.018.9107534
TCAGACC1136250.018.5150452
Produced by FastQC (version 0.11.5)