FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110975101
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1637360.14754300606583814No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1443840.13010486018841289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG626500.055.572422145
ACCGCGT296700.054.001936
CCGCGTT332450.048.09537
AGACCGC348700.045.7537734
CTCGCTA426900.042.201671
CAGACCG403600.040.1515733
TCGCTAT453750.039.0981942
CGCTATG485450.036.1828353
GCGTTCT467650.034.0239339
ACCGCTA159300.030.1956652
CTTCGAC875850.027.322079145
CGCGTTC597800.026.5501758
CGATCCT979750.025.591635145
GACCGCG673300.023.9535965
TCGCGTC179800.022.4631169
CCGCTAA243800.021.8224533
CTCGCGT195650.020.9806448
GTCGCGT49100.019.815431
CGTATCG116050.019.1115683
AGTCGCC361400.018.8254817
Produced by FastQC (version 0.11.5)