FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110975101
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA679550.032.0690884
TGCGACA368600.028.7497626
CGGAGGG824100.027.031051
GCGACAT399450.026.8936147
CAATCGC267350.026.3666271
TGGCGCG875900.025.1746644
TACCGTA174950.024.9776869
GGGCGAT246350.022.5586031
GGCGATC254500.022.4805132
TCAATCG191850.021.8530243
GAGGGCG1033650.021.3932533
GGGCGAG1066400.021.1371425
GCGAGGC1030950.021.1096217
CGACATC514300.021.0677578
CGGTGGC1421600.020.2739281
CGACCGT82500.020.2002621
GGCGAGG1137500.019.7830926
GTGGCGC1153900.019.6624033
CTACCGT227550.019.4649738
GCTGCGA558300.019.404344
Produced by FastQC (version 0.11.5)