FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127010101
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1303120.1025997137030857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG1307100.032.6963924
AGGGCGA810150.029.685494
TGCGACA461600.029.2680116
GCGACAT474050.028.7081157
GTGGCGC1670350.026.5282553
CAATCGC304950.026.0820331
CGGTGGC2409400.024.7639581
TCAATCG219600.023.6367993
CGACATC628600.021.7116788
CGTGCCT1935850.021.4299059
GGCGCGT1886250.021.3443015
CTGCGAC668650.020.5796225
GCTGCGA661000.020.5188354
GCGAGGC1189350.020.2375247
GGGCGAG1265150.019.6393685
GGGCGAT333800.019.327431
GGCGAGG1307250.019.0268426
TCGCGTC237900.018.7726049
CTCGCGT240800.018.546048
GCGTGCC2158450.018.3618328
Produced by FastQC (version 0.11.5)