FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103133316
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1670380.16196318171326907No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1542910.1496034511292161No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1393370.13510377189850076No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1317890.12778508934978877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG393850.046.252453145
CTCGCTA491550.045.798691
TCGCTAT526550.042.724932
CGCTATG554400.040.2406963
ACCGCGT260000.036.5718426
AGACCGC279900.033.468214
CCGCGTT285500.033.3276637
ACCGCTA160300.031.336122
CAGACCG343200.027.8833313
CTTCGAC591650.026.459612145
CGATCCT1165500.024.306631145
CCGCTAA227600.023.9688033
GCGTTCT395100.023.8025329
GCTATGT992000.022.9290354
CGCTAAG247750.021.0208824
CGTATCG64550.020.2709663
CCCGTAT151600.019.6031681
CGCGTTC480300.019.5667298
TCGTACG147300.018.43329210-14
CTATGTT1257800.018.3483525
Produced by FastQC (version 0.11.5)