FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103133316
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC230550.030.3708171
AGGGCGA679850.028.467974
CGGTGGC1376200.028.0839651
TGGCGCG821450.027.7745084
TCAATCG146950.027.4587333
CGGAGGG745350.026.178481
GCGAGGC875100.021.9747927
GGGGACC3618900.021.676846145
AATCGCC323400.021.5128942
GTGGCGC1123050.021.4474053
GCGACAT284650.021.0457697
GGTGGCG1692250.020.2441712
GGGCGAG982300.020.2397925
GGCGAGG1009900.019.7576436
GGCGCGT1088900.019.38985
GAGGGCG1016500.019.187293
TGCGACA325050.018.2490966
GGCGATC211950.018.0583932
GGGCGAT218050.018.0176531
CGTGCCT1289100.016.3930559
Produced by FastQC (version 0.11.5)