FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138052235
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2379230.17234273679089657No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1890810.13696337476897785No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1864550.13506119622040164No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1586600.11492751276355648No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1452710.10522900987441457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT532550.062.949356
CCGCGTT572350.058.6586277
AGACCGC601100.055.5650984
TCGACCG942650.054.868034145
CAGACCG638750.052.9306073
GCGTTCT782150.042.9240389
CGCGTTC801250.041.746388
CTCGCTA607200.040.440151
GACCGCG873900.038.5099565
GTGCTCT1359300.038.50932145
TCGCTAT678300.036.333362
CGCTATG736700.033.389023
ACCGCTA240150.033.0200842
TCAGACC1357350.026.392222
CCGCTAA328900.026.0852413
CTTCGAC1208200.025.105045145
CGATCCT1162750.025.047062145
CTCAGAC1742400.024.0790541
CGCTAAG388200.021.7491784
CGTATCG150550.020.7937723
Produced by FastQC (version 0.11.5)