FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138052235
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1833930.1328431951862279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT480500.028.568287
TGCGACA479550.028.4031166
CAATCGC315400.023.7779031
CGACATC627500.022.2896698
TCAATCG231500.021.4122963
GGGCGAT296100.021.0388981
AGGGCGA743150.020.1946624
TACCGTA196500.020.0973919
GGCGATC315600.019.538422
GCTGCGA712150.019.5104564
CTGCGAC751550.018.512455
CGGCCGC2502300.018.487305145
CGGTGGC1626050.018.3474861
CGGAGGG917900.017.1461
CTACCGT240950.016.7511888
GGGGACC3871850.015.683926145
AATCGCC485850.015.4737762
TGGCGCG1031650.015.2345144
CGATCTG458450.014.0140634
GTGGCGC1355350.013.9730663
Produced by FastQC (version 0.11.5)