FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105995768
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1643780.15507977639258202No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1113220.10502494778848151No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1071380.101077620381976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG475200.045.801506145
ACCGCGT304050.040.2022826
AGACCGC311550.038.7524264
CTCGCTA499600.038.625061
CCGCGTT332800.036.631637
TCGCTAT532750.035.995922
CAGACCG355400.034.4663283
CGCTATG569950.033.513253
ACCGCTA190550.030.051312
GCGTTCT438850.027.7647489
CGATCCT1159100.026.747547145
TCGCGTC254500.024.9623269
CTTCGAC680200.024.596405145
CTCGCGT270350.023.3783368
CCGCTAA267750.022.7893543
GTGCTCT935050.022.025846145
CGCGTTC566850.021.3227888
CGCTAAG295050.020.6586384
GCTATGT991350.019.5042744
TCGTACG169950.019.4996310-14
Produced by FastQC (version 0.11.5)