FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105995768
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG940000.026.6018734
CAATCGC221150.024.419871
CGGTGGC1480050.024.4049381
TCAATCG149450.024.2648053
GCGACAT306550.023.0838787
AGGGCGA749700.022.547094
GTGGCGC1265100.020.7728923
CGGAGGG834550.020.672451
GGCGCGT1186850.020.4524155
TGCGACA388450.018.4521756
GGTGGCG1774800.018.4245512
GGGGACC3740750.017.798285145
GCGAGGC942900.017.5044827
CGTGCCT1444650.017.2455229
AATCGCC317900.017.0237662
GGGCGAT231100.016.8932191
GCGTGCC1434600.016.7784298
GGGCGAG1058600.016.5356945
CGACATC449850.016.296258
ACGCCCG752600.016.2871346
Produced by FastQC (version 0.11.5)