FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109410541
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1523810.1392745146923275No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1369680.12518720659648325No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1255690.11476864921086535No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1129470.10323228362429905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT304450.046.2922866
CCGCGTT333450.042.423097
TCGACCG551000.042.09132145
CTCGCTA518150.040.0258561
AGACCGC369200.037.7662284
TCGCTAT555000.037.2577822
CAGACCG395850.035.603413
CGCTATG585500.035.392063
CTTCGAC776350.029.219982145
GCGTTCT487050.028.9226329
ACCGCTA160850.025.9386732
CGCGTTC563000.024.9047998
GTGCTCT1039900.023.231062145
GACCGCG616400.022.9493545
CGATCCT1252900.022.769594145
CCGCTAA206750.022.1006553
CGCTAAG216800.020.1716424
CGTATCG113100.019.5356353
GTCTCGC624400.019.399344
GCTATGT1078750.019.3608534
Produced by FastQC (version 0.11.5)