FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117067304
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2931780.2504354247365259No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2929550.2502449360241524No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2268950.19381585826901762No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2218710.18952430987904187No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT2056650.17568099116726904No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1923290.16428925364164873No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1626370.13892606598337653No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1443700.12332222154872552No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1436710.12272512912742913No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1403410.11988061158391417No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1380820.11795095238547562No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1287350.1099666564457656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT360200.047.073426
AGACCGC378750.044.2165454
CCGCGTT388350.043.5979967
TCGACCG530500.041.296005145
CTCGCTA866250.041.0566561
CAGACCG414100.040.990683
TCGCTAT998250.035.7570342
GTGCTCT1046150.034.68808145
GCGTTCT494950.034.021959
CGCTATG1096000.032.6222573
ACCGCTA205250.030.585652
CGATCCT2058100.028.51107145
CGCGTTC669150.025.1007638
CCGCTAA277800.024.4497933
GCTATGT1490650.024.2162134
CTTCGAC677050.023.656458145
GTCTCGC784850.023.207954
CGCTAGA274350.021.0496711
CTATGTT1763400.020.5890335
GACCGCG832200.020.4263465
Produced by FastQC (version 0.11.5)