FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117067304
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2335030.19946047446347615No Hit
CCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTA1405640.12007110029628769No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT1346770.11504236913152113No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG1226100.10473462342653762No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1201760.10265547757040686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC1832550.029.9044021
TCAATCG137650.029.22573
TGGCGCG1271850.027.8448454
CAATCGC206650.026.680511
GTGGCGC1634400.022.333843
AGGGCGA814550.021.6477764
GGGGACC5937450.020.737503145
GGTGGCG2273100.020.256462
GGCGCGT1685600.020.251555
CGGAGGG933650.019.7137361
CGGCCGC3029600.018.70667145
GCGACAT371750.018.700367
TGCGACA369100.018.6667636
AATCGCC306100.017.6958222
CGTGCCT1969200.017.689879
GCGTGCC1923150.017.5231868
GGCGATC260600.017.3251652
GGGCGAT278700.016.3238161
GGCGAGG1179750.015.5336156
GGGCGAG1197100.015.4377865
Produced by FastQC (version 0.11.5)