FastQCFastQC Report
Fri 22 Apr 2022
EGAF00005788153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132051155
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTA1799800.13629566511553798No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1524380.11543859650451373No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG1513410.11460785784115256No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA1369360.10369920656884826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC1755300.039.0477831
TGGCGCG1430000.032.2097974
GGCGCGT1528700.028.277715
GGTGGCG2091050.027.1976722
GTGGCGC1799550.026.697133
GCGTGCC1911550.022.2953518
CGTGCCT2074900.021.42139
GCGCGTG2061550.021.0381376
TCAATCG125750.020.9173243
GGGGACC7154950.020.815954145
CGCGTGC2072450.020.555397
CAATCGC188900.020.3025051
GGGCGAT258800.018.7430421
TGCGACA449150.017.6399466
TCGCGGC1050600.017.4387197
GGCGATC285050.017.3225542
ACGCCCG672050.015.583316
CCTCGCG1268350.015.1018745
AGGGCGA701950.014.8227944
CGGCCGC1889350.014.442505145
Produced by FastQC (version 0.11.5)