FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130552826
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1853850.14199999010362288No Hit
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1720000.13174743532552868No Hit
CCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTA1481580.11348509606371907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA1151000.036.753114
TCAATCG240950.035.190493
CAATCGC397900.034.7408521
CGGAGGG1550600.027.7898041
TGCGACA533450.027.044086
GCGACAT577950.025.2496597
GGCGATC331900.024.8417992
GGGCGAT331800.024.7652021
AATCGCC580900.023.9212
GGGCGAG1897700.022.6277795
GAGGGCG1908350.022.5226783
CGGTGGC2463300.022.2267671
GGCGAGG1961800.021.910656
TACCGTA190850.021.818979
TGGCGCG1600250.021.698764
GCGAGGC1927300.021.5646347
CGACATC754400.019.6133178
CGGCCGC6312250.018.856356145
GCTGCGA776450.018.7057424
TTAACGG115200.018.6683863
Produced by FastQC (version 0.11.5)