FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106286568
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1121030.10547240550659233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA661800.028.3686524
TGCGACA340650.025.8476776
GCGACAT359800.024.7517477
CGGAGGG888500.021.8789921
CAATCGC238150.021.2176281
TACCGTA193100.019.8493089
TCAATCG172950.019.0323893
GGGCGAT230450.018.602671
CGACATC499500.017.8921878
GAGGGCG1084500.017.845463
GGGCGAG1082050.017.8335195
GGCGATC245250.017.7600772
CTACCGT222850.017.3689278
GGCGAGG1131400.017.0500836
GCTGCGA533400.016.8495144
CGGCCGC3296500.016.698593145
TGGCGCG885500.016.6389164
CGGTGGC1501450.016.2267971
GCGAGGC1153800.016.1244347
AGGGATC640950.016.065972
Produced by FastQC (version 0.11.5)