FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117351413
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1378190.11744127870024028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC228450.021.8885571
TGGCGCG894100.020.7527054
GCGACAT273900.020.0856747
TGCGACA274650.019.8032536
TCGCGTC231250.019.0591269
CTCGCGT237000.018.6231088
TCAATCG168050.018.4518573
AGGGCGA688550.017.6777654
CGGTGGC1763950.017.614171
GTGGCGC1274400.015.6831683
AGGGATC720250.015.0437442
AATCGCC352250.014.0932162
GGGATCA757850.013.8257173
ATATCGG218250.013.672914145
GGCGCGT1254700.013.547725
CGACATC416500.013.5390228
GGTGGCG2041000.013.1356742
ACGCCCG963850.013.0566336
GGGGACC4295000.012.820383145
CTGCGAC446900.012.7159985
Produced by FastQC (version 0.11.5)