FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118953857
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG1016750.025.678044
CGGTGGC1645500.025.563771
TCGCGTC198600.022.6968259
CTCGCGT199350.022.6767758
AGGGCGA613900.021.3599554
GTGGCGC1314050.021.1711463
GCGACAT325250.020.507657
TGCGACA326200.020.187576
GGCGCGT1224100.019.54595
CAATCGC238650.019.4833091
GGTGGCG1913250.019.3555772
GGGGACC4814200.017.80343145
CGACCGT90950.016.920171
TCAATCG183900.016.5204473
GCGTGCC1480100.015.9112998
CGTGCCT1540000.015.8779479
GGGCGAG885600.015.4635035
GGGCGAT319100.015.0473181
GCGAGGC873500.015.0253477
TACCGTA209750.014.9809159
Produced by FastQC (version 0.11.5)