FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112161359
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1761840.15708083565570918No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1493500.13315637518265092No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1240540.11060315344431587No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1222300.10897692493187427No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1170110.10432380727483875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG729500.052.52734145
CTCGCTA360000.039.6442151
TCGCTAT383500.036.8762
CGCTATG402550.034.7076343
ACCGCGT228000.032.35016
CTTCGAC1009050.032.314537145
CCGCGTT256700.028.810767
AGACCGC261550.027.850574
TCGCGTC480250.026.9461569
CTCGCGT489300.026.4613198
ACCGCTA137900.025.0362132
CAGACCG304950.024.6265893
CGATCCT1023500.021.89301145
GCTCGCG683650.019.1522187
GTCTGGA1334400.019.111091
CCGCTAA208950.019.0500163
CGTATCG105050.019.0088963
GCGTTCT404650.018.4080229
CGCGTTC414000.017.6718528
CCCGTAT209200.017.5517981
Produced by FastQC (version 0.11.5)