FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112161359
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT300150.029.3775651
GGCGATC319450.027.803642
AGGGCGA705950.027.681974
GCGACAT477450.026.9406597
TGCGACA489950.026.1719676
TACCGTA156700.023.4887949
CGGTGGC1367550.023.3259751
TGGCGCG828250.022.134324
CGACATC594050.021.7414348
CGATCTG423550.021.3829234
GCGAGGC920650.021.1231617
CAATCGC265300.020.5292471
GGGCGAG1004500.020.0632935
GCTGCGA649150.019.9295184
GGCGAGG1039400.019.3490896
CGGAGGG1073250.019.1724341
GGGGACC3283350.018.798952145
CTGCGAC698550.018.5773835
TCGCGTC155550.018.323459
GTGGCGC1132350.018.296643
Produced by FastQC (version 0.11.5)