FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132956090
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1594160.11990123957465958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA941550.038.879974
CGGTGGC2384100.034.1690981
TGGCGCG1498900.030.2936634
GTGGCGC1846200.029.642223
GGGCGAG1398100.026.670765
GCGAGGC1391300.026.2101067
TACCGTA219050.025.8527059
GGGCGAT376000.025.0693511
CAATCGC333450.024.7447471
GGCGAGG1532200.024.3407966
GGTGGCG2837200.023.7919922
GGCGATC399400.023.4543952
GGGGACC6996150.022.727816145
TGCGACA442950.021.5021066
GCGACAT470450.020.5667447
CGGAGGG1957300.020.0705911
CTACCGT283600.019.9451758
GAGGGCG1938250.019.9152343
CGAGGCC1882200.019.8310498
TCAATCG231400.019.5967043
Produced by FastQC (version 0.11.5)