FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90227908
Sequences flagged as poor quality0
Sequence length89
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1482870.16434715520612536No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1408260.15607809503906483No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1373100.15218129627919558No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG918730.10182326293102129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT14149300.079.4075241
AGCAGTG16391800.068.502332
GCAGTGG21165400.053.0864373
CAGTGGT22035500.050.949734
AGTGGTA22772850.049.2812965
GTGGTAT24472050.045.8182836
TGGTATC24671250.045.46327
GGTATCA25183100.044.5868578
GTATCAA25255800.044.4792679
ATCAACG25255550.026.03011310-11
CAACGCA25275100.026.0248112-13
ACGCAGA25622900.025.6531214-15
TACATGG25302700.025.60664622-23
GCAGAGT25577150.025.59684816-17
AGTACAT25472050.025.51167120-21
AGAGTAC25777100.025.3407718-19
CATGGGA11174350.025.2289624-25
CATGGGG9749600.024.79829824-25
CATGGGC2914650.023.24775524-25
TCAACGC25254550.022.30347812-13
Produced by FastQC (version 0.11.5)