FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78628034
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTGA203050.019.8904361
CGTTGGG149850.018.618961
AGGGCGT105800.018.381753
GCGGTGA66050.018.3516925
GTAGAGG219850.018.155311
AGGGAAG121400.018.0674463
GGCTGTG152550.017.9793071
AGAGGAG98050.017.8601763
AGCCGGA110600.017.831698
GCGGAGC64500.017.752454
GACCGTG138700.017.7440361
TGCGGAG130800.017.719323
TGAAGGA190450.017.7017755
GTTGTCG182700.017.7001174
CGGAGCG44750.017.697295
CTGGACG119700.017.6101991
GCCCGAA149950.017.601381
GGAAGAG183150.017.59753
CAGGGCG90350.017.5462742
AGGGGTG63050.017.410564
Produced by FastQC (version 0.11.5)