FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154938622
Sequences flagged as poor quality0
Sequence length89
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1913890.12352568877242241No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1659930.10713468201621154No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1614470.10420061693849324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT7494750.069.026231
AGCAGTG7994550.064.6791462
GTGGTAT10155250.050.671036
GCAGTGG10221300.050.615443
CAGTGGT10380350.049.786754
AGTGGTA10405800.049.607755
TGGTATC10605350.048.500057
GTATCAA10671650.048.3282139
GGTATCA10749050.047.9656568
CAACGCA10240200.028.01255812-13
ATCAACG10315700.027.78982210-11
TACATGG10443300.026.83973922-23
GCAGAGT10770300.026.4679716-17
AGTACAT10818900.026.06706820-21
AGAGTAC10999350.025.84122718-19
ACGCAGA11302050.025.4467814-15
TCAACGC10225050.025.33721712-13
CATGGGG5203000.024.71008524-25
CGCAGAG10576000.024.52632116-17
GAGTACA10474750.024.49293320-21
Produced by FastQC (version 0.11.5)