FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100270383
Sequences flagged as poor quality0
Sequence length89
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1573220.1568977750887817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT12313650.076.030061
AGCAGTG13174300.071.017442
GCAGTGG15808550.059.2120933
CAGTGGT16305850.057.3650054
AGTGGTA16564100.056.4323045
GTGGTAT17074800.054.712586
GTATCAA17467950.053.5174069
TGGTATC17526500.053.320397
GGTATCA17587100.053.1637958
CAACGCA17233100.030.1337812-13
ATCAACG17262850.030.07127610-11
ACGCAGA17525650.029.62827114-15
TACATGG17349650.029.5312122-23
GCAGAGT17614850.029.40803316-17
AGTACAT17521950.029.32649820-21
CATGGGA10457550.029.15328424-25
AGAGTAC17722400.029.14322918-19
CATGGGG5572000.027.54962724-25
TCAACGC17206450.027.213712-13
CGCAGAG17378550.026.93676216-17
Produced by FastQC (version 0.11.5)