FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137362210
Sequences flagged as poor quality0
Sequence length89
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA38170902.778850165558635No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG19644481.4301225933974127No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCAGATGTGTATAAGAGAC11867040.8639231998378594No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAGAG9391190.6836807590675776No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA8695190.6330118014263166No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA5470490.39825291104445687No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAGTAC5418770.39448768333008044No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA4504690.3279424522945576No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG3283940.23907157579948662No Hit
GTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAA2703720.19683142838193998No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2479700.18052272164229158No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGTCAGATGTGTATAAGAGACAG2199220.16010371411467536No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2051460.14934675264761682No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAA1898340.13819958196653942No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAGAGT1729540.12591090373400368No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAGAGTA1647770.11995802921342048No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGTCAGATGTGTATAAGAGACA1629860.11865417715687598No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAAAAAAAAAAAAAAAAA1525410.11105019349936202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT32856500.069.65851
AGCAGTG36899650.062.0067182
GCAGTGG44854750.051.0064543
CAGTGGT45820450.049.8719334
AGTGGTA46587450.049.01225
GTGGTAT49771650.045.8347666
TGGTATC49829700.045.7404337
GGTATCA50662050.044.9920238
GTATCAA50868350.044.7977459
ACGACGC92000.029.4082345
CACGACG106550.025.2755364
ATCAACG51261950.025.14924810-11
CAACGCA51543150.025.02264212-13
ACGCAGA52115400.024.76049214-15
AGAGTAC52054700.024.58876218-19
GCAGAGT52267700.024.58740616-17
CATGGGG14524400.024.52879724-25
TACATGG51704550.024.4918422-23
AGTACAT52026850.024.4460420-21
CATGGGA31333050.024.10008424-25
Produced by FastQC (version 0.11.5)