FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89992971
Sequences flagged as poor quality0
Sequence length89
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1392780.1547654205126754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT16544800.079.543891
AGCAGTG18576050.070.82342
GCAGTGG23096950.056.9874733
CAGTGGT24154500.054.4327964
AGTGGTA24988900.052.600945
GTGGTAT26733550.049.138376
TGGTATC26876750.048.8679357
GGTATCA27464700.047.8514988
GTATCAA27544250.047.7237859
ATCAACG27593450.026.80567410-11
CAACGCA27624950.026.7851312-13
ACGCAGA27866850.026.54211414-15
GCAGAGT27967600.026.37217116-17
TACATGG27703250.026.3568822-23
AGTACAT27856000.026.30260520-21
AGAGTAC28064000.026.22965618-19
CATGGGA16153550.026.1956924-25
CATGGGG7573600.025.04308124-25
TCAACGC27615350.023.8668512-13
TGGGATA2198050.023.7245226-27
Produced by FastQC (version 0.11.5)