FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122146639
Sequences flagged as poor quality0
Sequence length89
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4195850.060.4437521
AGCAGTG4208800.060.1972162
GTGGTAT5239700.048.254826
GCAGTGG5404900.047.019783
AGTGGTA5399500.046.8943335
CAGTGGT5550050.045.7845464
GTATCAA5564200.045.460669
GGTATCA5778850.043.8295148
TGGTATC5899950.042.92917
CAACGCA5235250.027.28029412-13
ATCAACG5266900.027.14274410-11
TACATGG5449750.025.742122-23
GCAGAGT5670550.025.15355716-17
AGAGTAC5729450.024.7602218-19
ACGCAGA5866450.024.41126614-15
AGTACAT5791400.024.36748520-21
TCAACGC5218150.024.30899412-13
GAGTACA5373950.023.37484420-21
GTACATG5418200.023.06489422-23
TATCAAC5545550.022.88654710-11
Produced by FastQC (version 0.11.5)