FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103616826
Sequences flagged as poor quality0
Sequence length89
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1671250.1612913717314599No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1559570.15051319946820219No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1342680.1295812709028551No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1096940.1058650455091145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT8816950.075.051691
AGCAGTG9537200.069.3969962
GCAGTGG11846900.055.9968833
CAGTGGT12615800.052.4750824
AGTGGTA12905450.051.1818925
GTGGTAT14077750.046.9086046
TGGTATC14488750.045.5816357
GGTATCA14939350.044.228548
GTATCAA14952150.044.153749
CAACGCA14895400.024.61638312-13
ATCAACG14924550.024.56066510-11
TACATGG14911450.024.09080522-23
GCAGAGT15246150.023.9358716-17
ACGCAGA15335550.023.91096514-15
AGAGTAC15235050.023.83112118-19
AGTACAT15148150.023.79254220-21
CATGGGG8527100.023.13278824-25
ACGCTCT81050.022.8363518
CATGGGA3771000.022.35420624-25
TCAACGC14878150.022.28586812-13
Produced by FastQC (version 0.11.5)