FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79000070
Sequences flagged as poor quality0
Sequence length89
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3746950.069.924361
AGCAGTG4196450.062.4016042
AGTGGTA5509600.047.4775855
CAGTGGT5576950.046.988314
GCAGTGG5730150.045.846483
GTGGTAT5706700.045.757066
TGGTATC5923400.044.082717
GTATCAA6041300.043.2831549
GGTATCA6073900.043.034288
CAACGCA5788050.026.29650912-13
ATCAACG5856500.025.98878110-11
TACATGG5913900.025.24786622-23
GCAGAGT6047600.025.06071716-17
ACGCAGA6144550.024.79703314-15
AGTACAT6067150.024.73814820-21
AGAGTAC6366750.023.7368318-19
CATGGGG3215300.023.69542324-25
ACGACGC46650.023.664555
TCAACGC5825100.022.5131812-13
CATGGGA1839300.022.38749324-25
Produced by FastQC (version 0.11.5)