FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134148620
Sequences flagged as poor quality0
Sequence length89
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1677760.12506725749396452No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1669530.12445375882360922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT9061850.072.499231
AGCAGTG9881800.066.481522
GCAGTGG12269500.053.5843773
AGTGGTA12352350.053.061385
CAGTGGT12413500.052.882224
GTGGTAT12895750.050.781086
TGGTATC13363500.049.0282947
GTATCAA13500350.048.5739369
GGTATCA13566050.048.3308538
CAACGCA13114200.028.05199212-13
ATCAACG13135800.027.99240110-11
ACGCAGA13477950.027.29129814-15
TACATGG13265450.027.23829822-23
GCAGAGT13563850.027.00201216-17
AGTACAT13493450.026.87869820-21
AGAGTAC13662650.026.72120518-19
CATGGGG7508050.025.63566824-25
CATGGGA4360900.025.4112524-25
TCAACGC13104850.025.06111512-13
AACGCAG13389200.024.54073514-15
Produced by FastQC (version 0.11.5)