FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91899730
Sequences flagged as poor quality0
Sequence length89
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1006560.10952806934253234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5800400.072.608281
AGCAGTG6290100.066.9219362
GCAGTGG7880150.053.4982343
AGTGGTA7886800.053.303125
CAGTGGT7982700.052.756894
GTGGTAT7973700.052.658716
GTATCAA8284950.050.8021669
TGGTATC8315150.050.5342867
GGTATCA8393550.050.1310168
CAACGCA7945000.029.89347512-13
ATCAACG7998500.029.67892810-11
ACGACGC47550.028.974975
ACGCAGA8250250.028.78547914-15
TACATGG8125250.028.74641422-23
GCAGAGT8290450.028.53956816-17
CGACGCT47150.028.428656
AGTACAT8369300.028.04260820-21
AGAGTAC8423200.028.01768518-19
CATGGGA4078800.027.35876324-25
TCAACGC7956650.026.50748612-13
Produced by FastQC (version 0.11.5)