FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38800335
Sequences flagged as poor quality0
Sequence length89
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT6078550.078.8054351
AGCAGTG6746350.070.991012
GCAGTGG8223800.058.2717973
CAGTGGT8530750.056.1187064
AGTGGTA8722900.054.8373155
GTGGTAT9189850.052.030496
TGGTATC9333900.051.2218867
GTATCAA9407450.050.79989
GGTATCA9429000.050.708358
CAACGCA9399850.028.48564312-13
ATCAACG9406100.028.46936210-11
ACGCAGA9502700.028.16969314-15
GCAGAGT9558200.027.93875516-17
TACATGG9466700.027.92417322-23
AGTACAT9496400.027.89234220-21
AGAGTAC9601250.027.7143918-19
CATGGGA7391850.027.61082324-25
TCAACGC9393300.025.50790412-13
AACGCAG9510450.025.20021814-15
CGCAGAG9507300.025.19669516-17
Produced by FastQC (version 0.11.5)