FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133900298
Sequences flagged as poor quality0
Sequence length89
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT4397430.3284107702284576No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1533110.11449638446659768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT7940600.074.286631
AGCAGTG8838200.066.733792
GCAGTGG10802900.054.6353653
CAGTGGT11006000.053.5894474
AGTGGTA11215500.052.533285
GTGGTAT11444000.051.4386636
TGGTATC11790250.049.934457
GGTATCA12019800.049.023758
GTATCAA12054050.048.907699
ACGACGC65800.029.133945
CAACGCA11631350.028.44463312-13
ATCAACG11696600.028.2847710-11
TACATGG11749650.027.76026222-23
GCAGAGT11973400.027.55220416-17
ACGCAGA12207350.027.12046414-15
AGTACAT12119850.027.02546720-21
CATGGGA5467050.026.60552824-25
CATGGGG4717650.026.28949224-25
ACTTCGC853900.025.73937434-35
TCAACGC11650000.025.36838512-13
Produced by FastQC (version 0.11.5)