FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128704439
Sequences flagged as poor quality0
Sequence length89
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2989190.23225228463176784No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1314190.10210914325961983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT18260100.078.9832151
AGCAGTG19814000.072.7889252
GCAGTGG24201050.059.660783
CAGTGGT25143300.057.3289344
AGTGGTA25642000.056.1670845
GTGGTAT27790150.051.7894366
TGGTATC28162500.051.106587
GGTATCA28875150.049.8593988
GTATCAA28996750.049.651269
CAACGCA29007600.026.96289812-13
ATCAACG28999050.026.95335410-11
ACGCAGA29305150.026.67857414-15
TACATGG29071500.026.51641722-23
GCAGAGT29417550.026.49128516-17
AGTACAT29227550.026.4551220-21
CATGGGA17378850.026.33080124-25
AGAGTAC29618800.026.22884618-19
TCAACGC28980050.024.91384912-13
AACGCAG29293600.024.65946414-15
CGCAGAG29339650.024.6075916-17
Produced by FastQC (version 0.11.5)