FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130032902
Sequences flagged as poor quality0
Sequence length89
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT4382110.33700009248428525No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTCCGCCAGACCGCCGC1639530.12608578096642034No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG1570190.12075328442642924No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTCTGGTCTCGGCCGCA1514590.11647744353194547No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1473250.1132982481618383No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1465780.11272377817115856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT13474500.076.711551
AGCAGTG14393800.071.830342
GCAGTGG17415500.059.42723
CAGTGGT18544350.055.736974
AGTGGTA18949700.054.472735
GTGGTAT20884100.049.3949746
TGGTATC21577900.047.8182337
GTATCAA22446550.045.9626129
GGTATCA22495650.045.8830648
CAACGCA22577850.024.77790512-13
ATCAACG22579450.024.76686910-11
TACATGG22604250.024.29669822-23
ACGCAGA23052500.024.26967414-15
GCAGAGT23036350.024.17977716-17
AGTACAT22909250.024.07636820-21
AGAGTAC23045850.024.06915318-19
CATGGGG12313650.023.25826324-25
CATGGGA5749700.023.20471224-25
TATCAAC22513750.022.96798310-11
TCAACGC22556950.022.93722712-13
Produced by FastQC (version 0.11.5)