FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144339130
Sequences flagged as poor quality0
Sequence length89
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1966840.13626519710905838No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1821730.12621179024703835No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1600870.11091032625733575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT7384500.069.309461
AGCAGTG7831600.065.3094562
GCAGTGG9999350.051.224983
CAGTGGT10029500.050.992734
GTGGTAT10085850.050.5146266
AGTGGTA10336400.049.4166375
TGGTATC10423400.048.902317
GTATCAA10551900.048.4043739
GGTATCA10559550.048.3449758
CAACGCA10136900.028.07646812-13
ATCAACG10186650.027.91174910-11
ACGACGC62850.026.9394515
TACATGG10399450.026.83238422-23
GCAGAGT10671650.026.54935816-17
AGTACAT10778750.026.04432920-21
ACGCAGA11097150.025.69443314-15
AGAGTAC11124200.025.4014218-19
TCAACGC10126700.025.33301512-13
CATGGGG5198900.025.08986724-25
CGCAGAG10300100.024.9004716-17
Produced by FastQC (version 0.11.5)