FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140089540
Sequences flagged as poor quality0
Sequence length89
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1820970.1299861502864525No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1654920.1181330169261745No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1462670.10440965114169123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT6976400.069.138971
AGCAGTG7409000.065.058622
GCAGTGG9423200.051.1982653
CAGTGGT9447050.050.9909864
GTGGTAT9498400.050.553516
AGTGGTA9786800.049.2134675
TGGTATC9808250.048.9439057
GTATCAA9931150.048.463529
GGTATCA9948600.048.3568238
ACGACGC60800.029.14445
CAACGCA9543650.028.11972812-13
ATCAACG9596600.027.93601610-11
TACATGG9777400.026.8406822-23
GCAGAGT10040500.026.5532316-17
AGTACAT10137550.026.04534520-21
ACGCAGA10490800.025.64327214-15
CGACGCT68650.025.6304456
AGAGTAC10467400.025.41571618-19
TCAACGC9531750.025.38730812-13
CATGGGG4910750.025.06937624-25
Produced by FastQC (version 0.11.5)