FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135492548
Sequences flagged as poor quality0
Sequence length89
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG4032300.057.725842
AAGCAGT4037600.057.6826441
AGTGGTA4956050.046.810265
GTGGTAT4969350.046.5914576
CAGTGGT5199400.044.8024334
GCAGTGG5287800.044.165653
GTATCAA5313350.043.610829
GGTATCA5411800.042.8356748
TGGTATC5530200.041.95657
CAACGCA4914650.026.36056312-13
ATCAACG4968300.026.07683610-11
ACGACGC74850.025.5562065
TACATGG5183400.024.52725822-23
GCAGAGT5391700.023.96475216-17
ACGCAGA5439450.023.88108314-15
TCAACGC4917300.023.60889812-13
AGTACAT5530450.023.11151720-21
AGAGTAC5622550.022.86421418-19
GAGTACA5127100.022.4137920-21
CGCAGAG5225350.022.24049216-17
Produced by FastQC (version 0.11.5)