FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120932312
Sequences flagged as poor quality0
Sequence length89
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCAGATGTGTATAAGAGAC18440331.5248472219732307No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA16432481.3588163269383289No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG8256380.6827273756248041No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAGAG4622740.38225846538020375No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3150180.2604911746002177No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGTCAGATGTGTATAAGAGACAG2513270.20782452253124872No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAGTAC2140110.17696759158958278No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGTCAGATGTGTATAAGAGACA1812540.14988053813111585No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1445260.11950982959789937No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA1433340.11852415423927395No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1412790.1168248565362746No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA1295870.10715663816962336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT33078700.077.186661
AGCAGTG36360100.070.2033162
GCAGTGG42045300.060.706233
CAGTGGT43163950.059.0928344
AGTGGTA43814050.058.1975
GTGGTAT44999850.056.6383556
TGGTATC45183000.056.4014437
GTATCAA45441100.056.089919
GGTATCA45473200.056.0489438
ACGACGC49550.034.335745
ATCAACG45463900.030.85187110-11
CAACGCA45574050.030.79037712-13
ACGCAGA45910750.030.56630914-15
AGAGTAC45863900.030.45933218-19
GCAGAGT46035950.030.42162716-17
TACATGG45776050.030.3591522-23
AGTACAT45877900.030.34826920-21
CATGGGA32315600.030.26710724-25
CATGGGG10084400.029.74553924-25
TGGGATA2643250.028.55101226-27
Produced by FastQC (version 0.11.5)