FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121012725
Sequences flagged as poor quality0
Sequence length89
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA3560140.294195507125387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT25423050.079.86951
AGCAGTG28120600.072.196612
GCAGTGG33485400.060.6516953
CAGTGGT34352600.059.0701454
AGTGGTA34982750.057.9828575
GTGGTAT35942700.056.410486
TGGTATC36072900.056.1992347
GTATCAA36350750.055.785269
GGTATCA36368000.055.757298
ACGACGC65700.032.2111855
ATCAACG36369350.030.98670610-11
CAACGCA36464100.030.9231212-13
ACGCAGA36854600.030.59416814-15
AGAGTAC36795450.030.46198518-19
GCAGAGT36991550.030.40143616-17
TACATGG36733500.030.30568322-23
AGTACAT36874750.030.27056520-21
CATGGGA24333950.030.01626624-25
CATGGGG8756350.028.71492224-25
TCAACGC36383000.027.9400612-13
Produced by FastQC (version 0.11.5)