FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122107426
Sequences flagged as poor quality0
Sequence length89
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCAGATGTGTATAAGAGAC8535280.6989976187033866No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA3181280.2605312472969498No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2301630.18849222159510592No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGTCAGATGTGTATAAGAGACAG1376550.11273270144929595No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1337350.10952241348531906No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1311050.10736857232581415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT10891950.074.928791
AGCAGTG12286950.066.42062
GCAGTGG14542250.056.1555253
CAGTGGT14627650.055.7511634
AGTGGTA14727600.055.348015
GTGGTAT14995200.054.318786
TGGTATC14993050.054.3087
GGTATCA15094500.053.9485978
GTATCAA15161500.053.7107669
ACGACGC62100.030.9384045
ATCAACG15073850.030.19004610-11
CAACGCA15108850.030.12067212-13
ACGCAGA15452700.029.45026414-15
AGAGTAC15399450.029.36286418-19
CACGACG65600.029.2877244
GCAGAGT15506150.029.25536516-17
TACATGG15375400.029.23017522-23
AGTACAT15471950.029.10730220-21
CATGGGG6039150.028.13956324-25
CATGGGA7640200.027.63641224-25
Produced by FastQC (version 0.11.5)