FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94932998
Sequences flagged as poor quality0
Sequence length89
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCAGATGTGTATAAGAGAC3252470.34260689839374925No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2823400.2974097584066607No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1754370.1848008634468702No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG950760.10015063466130081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT6437100.069.8396761
AGCAGTG7134250.063.028822
GCAGTGG8507450.052.8987773
CAGTGGT8547700.052.5792124
AGTGGTA8581350.052.339665
GTGGTAT8604950.052.1285556
TGGTATC8700750.051.545187
GGTATCA8712150.051.476938
GTATCAA8748350.051.265829
ATCAACG8641800.028.95816810-11
CAACGCA8657150.028.91414612-13
TACATGG8824000.027.91347722-23
ACGCAGA9006050.027.82356514-15
AGAGTAC8932900.027.81030718-19
GCAGAGT9003300.027.70201716-17
AGTACAT9023200.027.40325420-21
CATGGGG3883000.026.39529424-25
TCAACGC8627850.026.05684912-13
ACGACGC51600.025.4927335
CGCAGAG8854400.025.39792616-17
Produced by FastQC (version 0.11.5)