FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93064472
Sequences flagged as poor quality0
Sequence length89
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCAGATGTGTATAAGAGAC3262790.3505945856545557No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2809240.30185955388002417No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1748290.18785794003107867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT6281350.069.807221
AGCAGTG6947000.063.1360932
GCAGTGG8277450.053.029083
CAGTGGT8334800.052.5903784
AGTGGTA8362750.052.3754355
GTGGTAT8382550.052.1789556
GGTATCA8472950.051.6058858
TGGTATC8474850.051.5927127
GTATCAA8522300.051.3158159
ATCAACG8413050.028.94410910-11
CAACGCA8420800.028.9318412-13
TACATGG8579900.027.93427822-23
ACGCAGA8758400.027.84513514-15
AGAGTAC8695100.027.82013918-19
GCAGAGT8769850.027.6938816-17
AGTACAT8780400.027.4041520-21
ACGACGC50950.026.9581455
CATGGGG3781350.026.5459724-25
TCAACGC8393700.026.12349512-13
CGCAGAG8611500.025.46521416-17
Produced by FastQC (version 0.11.5)