FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121051204
Sequences flagged as poor quality0
Sequence length89
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1324240.1093950292307708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT9334900.073.996671
AGCAGTG10236550.067.467982
GCAGTGG13028150.053.050523
CAGTGGT13429600.051.4177254
AGTGGTA13641000.050.5656135
GTGGTAT14166100.048.6362156
TGGTATC14515450.047.462587
GTATCAA14808150.046.5809339
GGTATCA14952550.046.1244778
CGACGCT130100.029.2466626
ACGACGC136200.028.6374915
CAACGCA14504800.026.86057712-13
ATCAACG14595150.026.69034210-11
ACGCTCT147150.026.1961338
TACATGG14572700.026.1446722-23
GCAGAGT14966750.025.8976216-17
AGTACAT14818450.025.86706520-21
ACGCAGA15098000.025.81865514-15
AGAGTAC14978550.025.77608518-19
CATGGGG7346250.024.94657524-25
Produced by FastQC (version 0.11.5)