FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121239009
Sequences flagged as poor quality0
Sequence length89
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1334990.11011224943285375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT9420600.074.035311
AGCAGTG10321050.067.527232
GCAGTGG13132150.053.1569443
CAGTGGT13533750.051.5400164
AGTGGTA13749400.050.665555
GTGGTAT14265350.048.7789736
TGGTATC14612000.047.6348237
GTATCAA14913550.046.7205169
GGTATCA15063500.046.248358
CGACGCT130150.029.203576
ACGACGC133100.028.8992335
CAACGCA14602600.026.98415412-13
ATCAACG14701300.026.79875610-11
ACGCTCT142500.026.7018538
TACATGG14693350.026.2521522-23
GCAGAGT15072950.026.00652316-17
AGTACAT14927700.025.98456620-21
ACGCAGA15205200.025.92633814-15
AGAGTAC15083550.025.90487518-19
CATGGGG7411950.025.08586124-25
Produced by FastQC (version 0.11.5)