FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38239796
Sequences flagged as poor quality0
Sequence length89
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5983600.078.652291
AGCAGTG6628700.070.974112
GCAGTGG8114650.058.015653
CAGTGGT8402600.055.9484064
AGTGGTA8599300.054.63685
GTGGTAT9050150.051.887456
TGGTATC9191700.051.0909047
GTATCAA9267650.050.656989
GGTATCA9283900.050.5803838
ATCAACG9264700.028.38135710-11
CAACGCA9266250.028.378412-13
ACGCAGA9365100.028.06676914-15
TACATGG9327500.027.8354122-23
GCAGAGT9421750.027.8346416-17
AGTACAT9362650.027.782120-21
AGAGTAC9466800.027.60117318-19
CATGGGA7262900.027.59839424-25
TCAACGC9255600.025.43902612-13
AACGCAG9368600.025.13891814-15
CGCAGAG9371000.025.11481516-17
Produced by FastQC (version 0.11.5)